I think I must bump this to address something VERY important: This game is now VERY popular, they are even making a SEQUEL:
And let me tell you about the characters:
Isaac
Magdalene (AKA The Virgin Mary)
Judas (When you begin the game as him, you get three coins. Hilarious.)
Eve (Unlocked by making 2 deals with SATAN)
Satan (The final enemy of the game)
Isaac's Mother (A Christian told to sacrifice her son and then killed)
And some of the items:
Brimstone
Pentagram
Blood Lust
Dead Bird
Whore of Babylon
ALL of these items are GOOD THINGS in the game. I think I've made my point.