The E. coli long-term evolution experiment was started in 1988 by evolutionary biologist Richard Lenski. One would think that after 25 years and over 55,000 generations, he would have been able to prove evolution by now. But his greatest finding so far is a group of bacteria that supposedly evolved the ability to digest citrate in the presence of oxygen.
In 2003 he allegedly noticed one of the flasks containing the bacteria had turned cloudy, a sign of population explosion. He claimed the bacteria in this flask were reproducing more rapidly because they had developed the ability to digest citrate, one of the ingredients in the microbial broth. It was claimed that this was not simply a case of natural selection enabling a species to do something better but to do something new. Lenski says he conducted a series of tests to determine when this mutation occurred, thawing out samples from his “fossil record” of frozen bacteria, taken every 500 generations. He claimed that the ability to digest citrate arose from the accumulation of several mutations. Lenski published his findings in 2008 (what took him so long?), after only 14 days of peer review, in the Proceedings of the National Academy of Sciences, which has a strong bias toward atheism and evolution. Last year Lenski published another paper supposedly detailing the actual mutations that occurred, which can be summarized as follows.
I have the following objections to Lenski’s findings – if they are true (and that’s a big “if”).
In short, all we have is Lenski’s word that some of his bacteria have evolved the ability to digest citrate in the absence of oxygen due to the accumulation of mutations over many generations. Lenski also claims other, less significant changes have taken place. The actual evidence, the bacteria themselves, are supposedly locked away in Lenski’s lab. He won’t share them with anyone. But why not, if his findings are accurate? What is he afraid of? Does he even have the bacteria? Here’s a picture of the colonies of E. coli involved in the experiment.

How do we know these flasks aren’t just filled with water? Do any of these flasks look cloudy to you? Over the years, many discoveries thought to support evolution (such as Piltdown Man and Nebraska Man and… well, that’s about it actually) have been proven to be fraudulent. Don’t be fooled by the long-term evolution experiment.
In 2003 he allegedly noticed one of the flasks containing the bacteria had turned cloudy, a sign of population explosion. He claimed the bacteria in this flask were reproducing more rapidly because they had developed the ability to digest citrate, one of the ingredients in the microbial broth. It was claimed that this was not simply a case of natural selection enabling a species to do something better but to do something new. Lenski says he conducted a series of tests to determine when this mutation occurred, thawing out samples from his “fossil record” of frozen bacteria, taken every 500 generations. He claimed that the ability to digest citrate arose from the accumulation of several mutations. Lenski published his findings in 2008 (what took him so long?), after only 14 days of peer review, in the Proceedings of the National Academy of Sciences, which has a strong bias toward atheism and evolution. Last year Lenski published another paper supposedly detailing the actual mutations that occurred, which can be summarized as follows.
Chapter One (from generaton zeo to at least generation 20,000): Our hero, E. coli, picks up mutations that don’t seem to have anything to do with feeding on citrate. They might have helped the bacteria grow better on their stingy rations of glucose. At least one of those mutations set the stage for feeding on citrate.
Chapter Two (around generation 31,500): The bacteria accidentally rewire their genome, so that a new copy of citT switches on in the presence of oxygen. Thanks to the mutations of Chapter One, this rewiring yields a modest but important improvement. Now the bacteria can feed a little on citrate, as well as on glucose.
Chapter Three (from about generation 31,500 to 33,000–and beyond): The bacteria make extra copies of the new and improved citT. They can pull in more citrate; new mutations fine-tune their metabolism to grow quickly on the molecule. World domination soon follows.
Chapter Two (around generation 31,500): The bacteria accidentally rewire their genome, so that a new copy of citT switches on in the presence of oxygen. Thanks to the mutations of Chapter One, this rewiring yields a modest but important improvement. Now the bacteria can feed a little on citrate, as well as on glucose.
Chapter Three (from about generation 31,500 to 33,000–and beyond): The bacteria make extra copies of the new and improved citT. They can pull in more citrate; new mutations fine-tune their metabolism to grow quickly on the molecule. World domination soon follows.
I have the following objections to Lenski’s findings – if they are true (and that’s a big “if”).
· No new functionality has been added to the cell.
· No new information has been added to the genome; there has only been a loss of pre-existing information.
· Perhaps only one mutation was needed to be able to digest citrate.
· Perhaps the ability to digest citrate was the result of a recessive gene.
· This still doesn’t explain how life came from non-life (abiogenesis).
· Mutations are never beneficial.
· Perhaps the population explosion was caused by contamination with another kind of bacteria that grows on citrate.
· Perhaps the sample was contaminated by another strain of E. coli previously unknown to science that could digest citrate.
· People like Dawkins and Coyne, who have written about Lenski’s findings, don’t define what they mean by “evolution,” whether they mean “microevolution,” or variation within a kind, which happens, or “macroevolution,” the origin of new species, which can’t happen.
· The bacteria are still bringing forth after their own kind, proving the Bible right (Gen 1:20). If evolution were true, a new species would have developed by now.
· As intelligent-design proponent Michael Behe said, “If the development of many of the features of the cell required multiple mutations during the course of evolution, then the cell is beyond Darwinian explanation" (The Edge of Evolution).
· There is a lot of missing data in Lenski’s 2008 paper. http://www.conservapedia.com/Richard_Lenski
· The findings of the study may well be fraudulent because Lenski refuses to give away samples of the bacteria so that Creationists can verify the results for themselves.
The Lenskigate affair began in mid-2008, when Andy Schlafly, founder of Conservapedia, wrote to Lenski politely asking him to supply the data for his findings. Lenski evaded Schlafly’s request, referring him to his website and to the paper itself. Much discussion ensued on the Conservapedia talk page. One man suggested Lenski should send Conservapedia some of the bacteria in the post so that they could conduct their own study. Schlafly ended up writing to Lenski again. Lenski’s rude, arrogant refusal to meet Schlafly’s demands can be found here: http://www.conservapedia.com/Conserv...:Lenski_dialog· No new information has been added to the genome; there has only been a loss of pre-existing information.
· Perhaps only one mutation was needed to be able to digest citrate.
· Perhaps the ability to digest citrate was the result of a recessive gene.
· This still doesn’t explain how life came from non-life (abiogenesis).
· Mutations are never beneficial.
· Perhaps the population explosion was caused by contamination with another kind of bacteria that grows on citrate.
· Perhaps the sample was contaminated by another strain of E. coli previously unknown to science that could digest citrate.
· People like Dawkins and Coyne, who have written about Lenski’s findings, don’t define what they mean by “evolution,” whether they mean “microevolution,” or variation within a kind, which happens, or “macroevolution,” the origin of new species, which can’t happen.
· The bacteria are still bringing forth after their own kind, proving the Bible right (Gen 1:20). If evolution were true, a new species would have developed by now.
· As intelligent-design proponent Michael Behe said, “If the development of many of the features of the cell required multiple mutations during the course of evolution, then the cell is beyond Darwinian explanation" (The Edge of Evolution).
· There is a lot of missing data in Lenski’s 2008 paper. http://www.conservapedia.com/Richard_Lenski
· The findings of the study may well be fraudulent because Lenski refuses to give away samples of the bacteria so that Creationists can verify the results for themselves.
In short, all we have is Lenski’s word that some of his bacteria have evolved the ability to digest citrate in the absence of oxygen due to the accumulation of mutations over many generations. Lenski also claims other, less significant changes have taken place. The actual evidence, the bacteria themselves, are supposedly locked away in Lenski’s lab. He won’t share them with anyone. But why not, if his findings are accurate? What is he afraid of? Does he even have the bacteria? Here’s a picture of the colonies of E. coli involved in the experiment.

How do we know these flasks aren’t just filled with water? Do any of these flasks look cloudy to you? Over the years, many discoveries thought to support evolution (such as Piltdown Man and Nebraska Man and… well, that’s about it actually) have been proven to be fraudulent. Don’t be fooled by the long-term evolution experiment.
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